Isifo i-Alzheimer's satholwa eminyakeni eminingi ngaphambi kokuba sitholwe

Isifo i-Alzheimer's satholwa eminyakeni eminingi ngaphambi kokuba sitholwe

Abacwaningi baye bakwazi ukusebenzisa inzwa esanda kuthuthukiswa ye-immunofluorescence infrared ukuze bathole ama-biomarker esifo se-Alzheimer kumasampula egazi iminyaka engu-17 ngaphambi kokuba kuvele izimpawu zomtholampilo, lapho kungenzeka khona ukuthola ukungalingani ekubunjweni kwe-beta-amyloid.

amadiphozithi ebuchosheni

Njengoba isifo se-Alzheimer siqhubeka, ukuphazamiseka kwephrotheni ye-amyloid-beta kubangela ukugaywa okuhlukile ebuchosheni, okubizwa ngokuthi ama-plaque, okungatholwa inzwa yemisebe eyakhiwe e-Bochum, eJalimane.

“Umgomo uwukuthola ingozi yokuba nesifo i-Alzheimer ngokuhamba kwesikhathi ngokuhlolwa kwegazi okulula ngisho nangaphambi kokuba kuvele ama-plaque anobuthi ebuchosheni, ukuze kuqinisekiswe ukuthi ukwelashwa kungaqala,” kusho uProfesa Klaus Gerwirt, umqondisi ongumsunguli we-PRODI. Centre for Protein Diagnostics at the Ruhr University in Bochum. Just in time”, njengengxenye yephrojekthi yocwaningo ngokubambisana neqembu lososayensi e-German Cancer Research Centre e-Heidelberg (DKFZ), eholwa nguProf Hermann Brenner.

Abacwaningi bashicilele imiphumela etholwe kusetshenziswa i-immunofluorescence infrared sensor kujenali i-Alzheimer's & Dementia, bechaza ukuthi lolu cwaningo lusekelwa ucwaningo oluqhathanisiwe olwanyatheliswa kumagazini ofanayo ngoMashi 2, 2022, olwenziwa abacwaningi besebenzisa i-molecule eyodwa ehambisanayo. i-array technology (SIMOA).

Ukonakala kokwakheka kwe-beta-amyloid nokugxila kwe-GFAP

Ithimba labacwaningi, eliholwa u-Klaus Gerwirt kanye no-Hermann Brenner, lifune ukuthola ukuthi ingabe kwakungenzeka ngempela yini ukuthola izimpawu zesifo i-Alzheimer kumasampula egazi. Eminyakeni engu-68 kamuva ngezinga eliphezulu lokunemba. Ukuze kuqhathaniswe, abacwaningi bahlole ezinye izimpawu zezinto eziphilayo besebenzisa ubuchwepheshe obuhambisanayo be-SIMOA - ikakhulukazi i-biomarker P-tau17, okwamanje ehlongozwayo njengekhandidethi elithembisayo le-biomarker ezifundweni ezihlukahlukene.

Ngokumangalisayo, abacwaningi bathi, "sithole ukuthi i-glial fiber protein GFAP ingabonisa isifo kuze kube yiminyaka engu-17 ngaphambi kwesiteji somtholampilo, nakuba inembile kakhulu kunenzwa ye-infrared ye-immune."

Kodwa abacwaningi bakwazi ukukhulisa ukunemba kokuhlolwa esigabeni esingenazimpawu zesifo i-Alzheimer ngokuhlanganisa ukuphazanyiswa kokwakheka kwe-amyloid beta nokugxila kwe-GFAP.

ngaphambi kokuba kwenzeke umonakalo

Abacwaningi bakwa-Bochum banethemba lokuthi ukuhlonzwa kusenesikhathi okusekelwe ku-amyloid-beta dysgenesis kuzosiza ukusetshenziswa kwezidakamizwa zesifo i-Alzheimer kusenesikhathi, ukuthi kuzoba nomthelela ongcono kakhulu.

"Sihlela ukusebenzisa ukuhlolwa kwe-dysprotein ukuze sisungule indlela yokuhlola abantu abadala asebekhulile ukuze banqume ubungozi babo bokuwohloka komqondo kwe-Alzheimer," kusho u-Klaus Gerwirt, ukuze ukwelashwa kuqale ngaphambi kokuba "umonakalo ongenakulungiseka."

"Kuze kube manje, inqwaba yokuhlolwa kwezidakamizwa ze-Alzheimer's kuhlulekile, ngokusobala ngenxa yokuthi ukuhlolwa kokuhlola uqweqwe lobuchopho olusetshenziswa ocwaningweni akusiboni lesi sifo kusenesikhathi," uyanezela uGerwirt, echaza ukuthi "uma ama-plaque esefakiwe, adala umonakalo ongalungiseki. "Kusengqondweni yesiguli."

Ukuxilongwa kwangaphambi kwesikhathi nangaphezulu

Ezivivinyweni ezisetshenziswa kuze kube manje, ama-plaque atholwa ngqo ebuchosheni kusetshenziswa ubuchwepheshe obuyinkimbinkimbi futhi obubizayo bokuskena i-PET noma akhonjwe ngokungaqondile ngendlela eyinkimbinkimbi kusetshenziswa ukugxilisa kweprotein biomarker ku-cerebrospinal fluid etholwe ngokuhlaselayo kusetshenziswa i-ELISA noma i-mass spectrometry.

Ngokuphambene nokuxilongwa kwe-plaque okusekelwe kahle, inzwa ye-infrared immune ibonisa ukungavamile kwangaphambilini ku-amyloid-beta, okungabangela ukufakwa kwe-plaque kamuva.

izimpawu ezibalulekile

Ukungajwayelekile kwamaprotheni kudlala indima enkulu ezifweni eziningi ze-neurodegenerative, njengesifo sikaParkinson, isifo sikaHuntington kanye ne-ALS.

Njengoba abacwaningi bechaza, inzwa ye-immuno-infrared ingasetshenziswa futhi ngokuyisisekelo ukuthola amanye amaprotheni aphethwe yi-denatured, njenge-TDP-43, eyenzeka ezigulini ze-ALS, ephawula ukuthi inzwa ayilinganisi ukuhlushwa kweprotheni ethile, kodwa kunalokho. ithola ukungalingani kusetshenziswa amasosha omzimba athile.

Iphuzu elibaluleke kakhulu, uGerwirt uyagcizelela, ukuthi "ubuchwepheshe bepulatifomu buvumela ukuxilongwa okuhlukile nokunembile okusekelwe ku-biomarker ezigabeni zokuqala zezifo ze-neurodeergenerative, lapho ukuxilongwa okusekelwe ezimpawu okwamanje okusetshenziswayo kunzima kakhulu futhi kujwayele ukuba namaphutha."